Daniel C. Volke

This paper introduces a rapid HPLC-based method to measure NAD(P)(H) and NMN redox cofactor concentrations and ratios in microbes, validated against a classical assay and applied to engineered E. coli and Pseudomonas putida.

This study optimizes DNA template design, reaction composition, and lysate genotype to extend CFPS reaction lifetime in a high-throughput format, achieving >14 h productivity and high deGFP yield.

This study investigates how Pseudomonas putida uses two transhydrogenases to maintain redox balance across different metabolic conditions, revealing their essential roles in growth and stress tolerance.

This paper introduces a growth-coupled biosynthesis strategy (GrowBio) for producing xanthommatin, demonstrating its effectiveness in microbial production through engineered Pseudomonas putida.

The paper uses experimental evolution to show that circadian clock mutations in cyanobacteria enhance growth under continuous light by altering transcriptomic and metabolic responses.

This paper enhances fluorinase catalytic efficiency by engineering mutants and optimizing reaction conditions, achieving a 60-fold increase in turnover rate.

This study disrupts all 32 GGDEF domain-containing proteins in P. aeruginosa to analyze c-di-GMP signaling, revealing residual c-di-GMP levels and the importance of functional overlap in bacterial signaling.

This paper introduces a CRISPR-Cas3-based genome engineering system for Pseudomonas species that enables efficient genomic integration and vector curing.

This study uses D-fluxomics to reveal the dynamic and hierarchical metabolism of glucose in Pseudomonas putida through the periplasmic gluconate shunt.

The paper identifies a novel archaeal fluorinase with the highest reported fluorination rate and demonstrates its application in enhancing fluoronucleotide production in bacterial hosts.

The paper analyzes G6PDH isozymes in Pseudomonas putida to show how cofactor specificity and gene duplication contribute to redox balance and metabolic strategies in bacteria.