Structural and Dynamic Impacts of Single-atom Disruptions to Guide RNA Interactions within the Recognition Lobe of Geobacillus stearothermophilus Cas9
Helen B Belato, Alexa L Knight, Alexandra M. D’Ordine, Chinmai Pindi, Zhi-Qiang Fan, Jinping Luo, Giulia Palermo, G. Jogl, George P. Lisi
This study investigates how single-atom mutations in the recognition lobe of GeoCas9 affect its structure, dynamics, and guide RNA interactions without altering its functional specificity.
eLife · Jul 16, 2024Peer-reviewedBiophysics