Author

Alexa L Knight

Brown UniversityORCID 0000-0002-5407-0157Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:protein folding & dynamics· 3 Organism:bacteria (other)· 1 Approach:biophysical modeling· 1 Approach:mechanistic / dynamical model· 1 Assay:protein structure· 1 Assay:structural biology· 1

Frequent Co-Authors

George P. LisiiD· 3 shared Giulia PalermoiD· 3 shared Charlotte S Dresser· 2 shared Chinmai PindiiD· 2 shared G. JogliD· 2 shared Helen B BelatoiD· 2 shared Jinping LuoiD· 2 shared Aakash SahaiD· 1 shared

Activity by Year

2024-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 37 mutual co-author links

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3 shared papersBiophysics

George P. LisiORCID

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3 papers

Recent·Most cited

A Cryptic Binding Pocket Regulates the Metal-Dependent Activity of Cas9

Mohd Ahsan, Alexa L Knight, Aakash Saha, Delisa Ramos, Isabel Strohkendl, Erin Skeens, M. Stewart West, Jinping Luo, Charlotte S Dresser, David Ward Taylor, George P. Lisi, Giulia Palermo

This study identifies a cryptic divalent metal binding pocket that regulates Cas9's metal-dependent activity through conformational activation and catalytic efficiency.

Nature Communications · Oct 9, 2026Peer-reviewedBiophysics

Orthosteric and allosteric effects of anti-CRISPR II-C1 inhibition on GeoCas9 from integrated structural biophysics

Alexa L Knight, Helen B Belato, Charlotte S Dresser, Chinmai Pindi, Briana J. Mercado, Praise Lasekan, Jinping Luo, Pablo Ricardo Arantes, G. Jogl, Giulia Palermo, George P. Lisi

This study investigates how AcrIIC1 inhibits GeoCas9 by analyzing structural and dynamic changes in the HNH nuclease domain.

bioRxiv · Apr 9, 2026 · PreprintBiophysics

Structural and Dynamic Impacts of Single-atom Disruptions to Guide RNA Interactions within the Recognition Lobe of Geobacillus stearothermophilus Cas9

Helen B Belato, Alexa L Knight, Alexandra M. D’Ordine, Chinmai Pindi, Zhi-Qiang Fan, Jinping Luo, Giulia Palermo, G. Jogl, George P. Lisi

This study investigates how single-atom mutations in the recognition lobe of GeoCas9 affect its structure, dynamics, and guide RNA interactions without altering its functional specificity.

eLife · Jul 16, 2024Peer-reviewedBiophysics