Optimised CRISPR-Cas9 mediated single molecule imaging for accurate quantification through endogenous expression.
Abdullah O. Khan, Carl W. White, Jeremy A. Pike, Jack Yule, Alexandre Slater, Stephen J. Hill, Natalie S. Poulter, Steven G. Thomas, Neil V. Morgan
This study optimizes CRISPR-Cas9 for single molecule imaging by improving mEos variants and shows endogenous labeling enables accurate quantification of CXCR4.
bioRxiv · Nov 30, 2018 · PreprintCell Biology