Molecular BiologybioRxivPreprint
High temperature ligation enables in vitro assembly of eukaryotic genes
Noah Evan Robinson, Bryan Gerber, Weilin Zhang…·Kaihang Wang
This paper introduces High Temperature (HighT) assembly, a DNA assembly method that improves accuracy and yield by ligating DNA at higher temperatures, and demonstrates its application in constructing synthetic genes and genomic regions.
DOI 10.64898/2026.10.09.758000