Kaihang Wang

The paper develops SPARTA for in situ genetic reprogramming of native microbiomes and introduces ScRibe-seq to identify engineered taxa, demonstrating functionality across diverse environments.

This paper introduces ACE, a method to create genomic chimeras by integrating large genomic segments from donor cells into a recipient genome, demonstrating its ability to generate stable chimeric organisms across species barriers.

This paper introduces High Temperature (HighT) assembly, a DNA assembly method that improves accuracy and yield by ligating DNA at higher temperatures, and demonstrates its application in constructing synthetic genes and genomic regions.

This paper introduces SLIM, a method for unbiased genome reduction in diverse bacteria, and demonstrates its application in Escherichia coli and other species.

The paper introduces a method for high-fidelity, multiplexed DNA construction from oligo pools, enabling efficient assembly of multiple defined constructs.

This study develops a rapid DNA template synthesis method called Sidewinder to produce polyadenylated Hantavirus mRNA vaccine candidates in just 2 days.

This paper demonstrates that CbAgo-mediated DNA cleavage in E. coli is mutagenic and can be used for genetic manipulation.

This paper introduces a CRISPR-associated transposon system for efficient linear DNA integration in E. coli, bypassing traditional limitations through oligonucleotide design and nested PCR.