Naoyuki Sugiyama

UniScore integrates and standardizes outputs from multiple search engines in proteomics, outperforming single search engines in large-scale data analysis.

The paper introduces a motif-centric phosphoproteomics approach to quantify phosphorylation pathways using in vitro kinase reactions and isobaric labeling, demonstrating selective quantification of over 7,000 tyrosine phosphorylation sites.

This study identifies endogenous kinase substrates using proximity labeling and phosphoproteomics, finding 33 CK2 and 52 PKA substrates and linking mutations to altered phosphorylation.