Yit‐Heng Chooi

The paper develops improved AMA1-based plasmids for more consistent and stronger gene expression in filamentous fungi, showing increased reporter expression, CRISPRa efficiency, and metabolite production.

CAGECAT enables rapid homology searches and visualization of gene clusters across genomes, aiding in the discovery of novel metabolites through comparative analysis.

The paper develops a system for targeted chromosomal integration of biosynthetic gene clusters in Aspergillus nidulans using Cre/lox-mediated recombination, demonstrating efficient integration and expression of a 21 kb DNA fragment.

clinker aligns translated genes from GenBank gene clusters, orders clusters by homology-plus-synteny similarity, and renders interactive, editable comparison figures via the D3-based clustermap.js library.

cblaster identifies and visualizes homologous gene clusters across local and remote databases, enhancing comparative genomics analysis.

A dLbCas12a-VPR CRISPR activation system works in Aspergillus nidulans, boosting microperfuranone output from the native micA gene and revealing dehydromicroperfuranone as the mic cluster product.

This study identifies secondary metabolite clusters in three wheat pathogens, showing horizontal gene acquisition and loss linked to host adaptation.