BiochemistryScientific ReportsPeer-reviewed
Absolute quantification of rare gene targets in limited samples using crude lysate and ddPCR
Charandeep Kaur, Stuart Paul Adams, Catherine N. Kibirige, Becca Asquith
This paper develops and validates a crude lysate ddPCR assay for accurate absolute quantification of rare genes like TRECs from limited clinical samples, eliminating DNA extraction and improving performance over standard ddPCR.
3 citations