Synthetic BiologybioRxivPreprint
Towards self-regeneration: exploring the limits of protein synthesis in the PURE cell-free transcription -translation system
Ragunathan Bava Ganesh, Sebastian J. Maerkl
The paper improves the PURE cell-free system by reducing non-ribosomal protein concentration and increasing synthesis efficiency, enabling self-regeneration of all 36 non-ribosomal proteins.
DOI 10.1101/2024.04.03.587879