Molecular BiologyRNA BiologyPeer-reviewed
An efficient, scarless, selection-free technology for phage engineering
Moran G. Goren, Tridib Mahata, Udi Qimron
The paper describes a method for efficiently engineering phage genomes without using selection or CRISPR-Cas systems, achieving mutation rates of 1-14% for single-base substitutions, deletions, and insertions in three phages.
7 citations