Scalable and Automated CRISPR-Based Strain Engineering Using Droplet Microfluidics
Kosuke Iwai, Maren Wehrs, Megan E. Garber, Jess M. Sustarich, Lauren Washburn, Zachary Costello, Peter W. Kim, David Ando, William R. Gaillard, Nathan J. Hillson, Paul D. Adams, Aindrila Mukhopadhyay, Héctor García Martín, Anup K. Singh
The paper describes a microfluidic system for high-throughput CRISPR-based gene editing and screening, demonstrating its ability to perform parallel genetic modifications in E. coli.
Microsystems & Nanoengineering · Mar 15, 2022Peer-reviewedSynthetic Biology