Christoph K. Spahn

The paper develops a platform for optimizing DNA-PAINT in Gram-negative bacteria, enabling high-quality 3D imaging of multiple targets in E. coli and Yersinia enterocolitica.

This study shows Yersinia enterocolitica suppresses T3SS expression and adhesion at high cell densities through VirF downregulation and CsrBC-mediated mechanisms.

This study shows that activating the Yersinia type III secretion system causes large-scale DNA rearrangements in both the virulence plasmid and chromosomal DNA.

Autoencoder design plus a roughly 24-hour cell-free screen tested 500 candidates over five rounds, finding 30 functional AMPs; six were broad-spectrum with no resistance emerging in E. coli.

ZeroCostDL4Mic provides a platform to use deep learning in microscopy, enabling tasks like segmentation and denoising through Google Colab.