Brooke E. White

Glycosomal phosphoenolpyruvate carboxykinase CRISPR/Cas9-deletion and its role in Trypanosoma cruzi metacyclogenesis and infectivity in mammalian host

This study uses CRISPR/Cas9 to delete PEPCK in T. cruzi, showing it impairs differentiation and host invasion but enhances replication once inside host cells.

Serial "deep-sampling" PCR of fragmented DNA reveals the wide range of Trypanosoma cruzi burden among chronically infected hosts and allows accurate monitoring of parasite load following treatment

This study introduces deep-sampling PCR to detect and quantify T. cruzi parasite load in chronically infected hosts, revealing large variations in parasite levels and enabling treatment monitoring.