A generalisable method for the purification and biophysical characterisation of bacterial membrane receptors
Federico Bosetto, Maria Zacharopoulou, Mark Bycroft, Monika Kish, Giovanna Zinzalla, Pamela J. E. Rowling, Stephen H. McLaughlin, Jonathan J. Phillips, Laura S. Itzhaki, Ioanna Mela
This paper describes a method to purify and characterize soluble domains of bacterial membrane receptors, demonstrating its effectiveness with the HasR receptor from Pseudomonas aeruginosa.
bioRxiv · Aug 12, 2026 · PreprintBiophysics