Heritable Large Deletions Using Type I-E CRISPR-Cas3 in Rice
Hiroaki Saika, Naho Hara, Shuhei Yasumoto, Toshiya Muranaka, Kazuto Yoshimi, Tomoji Mashimo, Seiichi Toki
This study demonstrates the use of Eco CRISPR-Cas3 in rice to generate heritable large deletions, achieving deletion frequencies of 21-61% and stable inheritance in progenies.
bioRxiv · Jun 5, 2025 · PreprintPlant Biology