Kazuto Yoshimi

This paper develops a CRISPR-Cas3-based diagnostic platform for emerging RNA viruses, enabling rapid assay design and deployment with high sensitivity and portability.

This study demonstrates that CRISPR-Cas3 can efficiently and safely disrupt genes in human T cells, reducing off-target effects and alloreactivity while maintaining cytotoxicity.

This paper develops a miRNA detection system combining TWJ-SDA, Multistep L-TEAM, and CRISPR-Cas3, achieving high sensitivity for low-concentration miRNA detection.

This study demonstrates the use of Eco CRISPR-Cas3 in rice to generate heritable large deletions, achieving deletion frequencies of 21-61% and stable inheritance in progenies.

This study elucidates the mechanism of CRISPR-Cas3-mediated DNA degradation in E. coli, showing how EcoCas3 cleaves ssDNA and degrades dsDNA through specific and non-specific interactions with EcoCascade.

The paper re-evaluates the efficiency of a CRISPR-Cas9 method for generating conditional knockout alleles in mice, finding very low success rates and high rates of undesired alleles.