Tomoji Mashimo

This paper develops a CRISPR-Cas3-based diagnostic platform for emerging RNA viruses, enabling rapid assay design and deployment with high sensitivity and portability.

This study demonstrates that CRISPR-Cas3 can efficiently and safely disrupt genes in human T cells, reducing off-target effects and alloreactivity while maintaining cytotoxicity.

This paper develops a non-invasive CG assay for detecting ovulation and early pregnancy in marmosets using monoclonal antibodies, achieving 75% embryo recovery through artificial insemination.

This paper develops a miRNA detection system combining TWJ-SDA, Multistep L-TEAM, and CRISPR-Cas3, achieving high sensitivity for low-concentration miRNA detection.

This study demonstrates the use of Eco CRISPR-Cas3 in rice to generate heritable large deletions, achieving deletion frequencies of 21-61% and stable inheritance in progenies.

The paper shows that opioidergic signals in the medial prefrontal cortex activate the descending pain inhibitory system to initiate placebo analgesia in neuropathic pain rats.

This study introduces a G51D mutation in the rat Snca gene, leading to synaptic mislocalization of α-synuclein and increased susceptibility to Lewy-like pathology.

This study uses 18F-DOPA PET imaging to identify a dopaminergic deficit in a rat model with a G51D α-synuclein mutation, showing reduced dopamine turnover and striatal asymmetry in aged animals.

The paper characterizes TH-Cre knock-in rats generated via Combi-CRISPR, showing cell type-specific Cre expression and normal catecholamine metabolism and behavior.

This study elucidates the mechanism of CRISPR-Cas3-mediated DNA degradation in E. coli, showing how EcoCas3 cleaves ssDNA and degrades dsDNA through specific and non-specific interactions with EcoCascade.

The paper introduces a DIO/USS system to enhance Cre-dependent transgene expression specificity by preventing Cre-independent recombination during vector production.

This study identifies a Txn1 mutation causing epilepsy and midbrain vacuolar degeneration in rats, with age-dependent neuronal and oligodendrocyte cell death and repair.