Context-Seq: CRISPR-Cas9 Targeted Nanopore Sequencing for Transmission Dynamics of Antimicrobial Resistance
Erica R. Fuhrmeister, Sooyeol Kim, Shruteek A. Mairal, Caroline E. M. McCormack, Benard Chieng, Jenna M. Swarthout, Abigail Harvey Paulos, Sammy M. Njenga, Amy J. Pickering
The paper introduces Context-Seq, a CRISPR-Cas9 targeted nanopore sequencing method to study AMR transmission between humans and animals, identifying shared ARG clusters and potential pathogenic hosts.
Nature Communications · Jul 3, 2025Peer-reviewedMicrobiology