Cell BiologybioRxivPreprint
In-Chamber Sublimation: A Practical Approach for Mitigating Ice and Curtaining in Cryo-Electron Tomography Lamellae Preparation
Amy L. Bondy, Genís Valentín Gesé, Thomas Thersleff, B. Martin Hällberg
This paper demonstrates that sublimation within the SEM chamber reduces ice contamination and curtaining in cryo-ET lamellae preparation without compromising vitrification.