Sarah I. Hernandez

This study shows that using a single plasmid for co-transfection is more efficient and results in tighter gene expression correlation than using multiple plasmids.

The paper introduces self-documenting plasmids that encode their own information in DNA, enabling verification without prior knowledge of the plasmid identity.

The paper evaluates sequencing strategies for accurate plasmid assembly, finding that hybrid approaches provide the most reliable results while highlighting challenges in GC-rich and repetitive regions.