Efficient DNA base editing via an optimized DYW-like deaminase
Jiyeon Kweon, Soomin Park, Mi Yeon Jeon, Kayeong Lim, Gayoung Jang, An-Hee Jang, Minyoung Lee, Cheong Seok, Chaeyeon Lee, Subin Park, Jiseong Ahn, JiYoon Jang, Young Hoon Sung, Daesik Kim, Yongsub Kim
This paper introduces SsCBE, a CRISPR-based cytosine base editor with improved efficiency, specificity, and reduced cytotoxicity, enabling targeted edits in mouse zygotes and mitochondrial DNA.
bioRxiv · May 15, 2024 · PreprintMolecular Biology