Gastón Courtade

NMR assignment and functional characterization of biotechnologically modified xanthan

This study provides a near-complete NMR assignment of xanthan gum and investigates how side-chain modifications affect its structural stability.

Decoding CBM2 binding modes: how similar folds achieve distinct substrate preferences

This study investigates how CBM2 proteins achieve distinct substrate preferences through a balance of topological complementarity, hydrophobic dehydration, and hydrogen-bonding interactions.

Conformation-Dependent Donor Selectivity in the Xanthan Gum Glycosyltransferase GumK Revealed by AI-Based Docking

This study uses AI-based docking and cofolding to show that GumK's donor selectivity depends on conformational states and ligand interactions, not a single rigid binding mode.

Comparative study of two xanthan gum glycosyltransferases combining AI structure predictions and molecular modeling

This study uses AI and molecular modeling to analyze the structure and substrate-binding of two xanthan gum glycosyltransferases, revealing differences in their catalytic mechanisms and membrane interactions.

Molecular basis of protein-glycan cross-linking by CpCBM92A revealed by NMR spectroscopy

This study uses NMR spectroscopy to reveal how the trivalent binding sites of CpCBM92A interact with beta-glucan ligands, showing distinct roles and affinities for cross-linking.

Computational characterization of the xanthan gum glycosyltransferase GumK

This paper uses computational methods to study GumK, a glycosyltransferase, revealing its dynamic behavior and substrate specificity mechanisms.

The C-terminal intrinsically disordered region of a fungal LPMO binds copper and displays anti-fungal properties

This study identifies the C-terminal disordered region of an AA14 LPMO as a copper-binding domain with antifungal activity.

Development of a Vibrio natriegens-based plate-clearing assay for rapid screening of PET-hydrolyzing enzymes

The paper develops a Vibrio natriegens-based plate-clearing assay to rapidly screen PET-hydrolyzing enzymes, showing improved degradation efficiency with a variant of FsC.

Standard Intein Gene Expression Ramps (SIGER) for protein-independent expression control

The paper introduces the SIGER system to control protein expression by decoupling translation initiation features from target genes using inteins, improving protein yield and quality.