Molecular BiologybioRxivPreprint
An Inexpensive RT-PCR Endpoint Diagnostic Assay for SARS-CoV-2 Using Nested PCR: Direct Assessment of Detection Efficiency of RT-qPCR Tests and Suitability for Surveillance
Jayeshkumar Narsibhai Davda, Keith Frank, Sivakumar Prakash…·Imran Siddiqi
The paper develops an inexpensive RT-nPCR assay for SARS-CoV-2 that matches RT-qPCR performance and highlights low detection efficiency in real-world RT-qPCR testing.
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