An Inexpensive RT-PCR Endpoint Diagnostic Assay for SARS-CoV-2 Using Nested PCR: Direct Assessment of Detection Efficiency of RT-qPCR Tests and Suitability for Surveillance
Jayeshkumar Narsibhai Davda, Keith Frank, Sivakumar Prakash, Gunjan Purohit, Devi Prasad Vijayashankar, Dhiviya Vedagiri, Karthik Bharadwaj Tallapaka, Krishnan Harinivas Harshan, Archana Bharadwaj Siva, Rakesh K. Mishra, Jyotsna Dhawan, Imran Siddiqi
The paper develops an inexpensive RT-nPCR assay for SARS-CoV-2 that matches RT-qPCR performance and highlights low detection efficiency in real-world RT-qPCR testing.
bioRxiv · Jun 8, 2020 · PreprintMolecular Biology