Systematic reconstruction of molecular pathway signatures using scalable single-cell perturbation screens
Longda Jiang, Carol Dalgarno, Efthymia Papalexi, Isabella Mascio, Hans‐Hermann Wessels, Huiyoung Yun, Nika Iremadze, Gila Lithwick‐Yanai, Doron Lipson, Rahul Satija
The paper uses Perturb-seq to systematically identify signaling regulator targets across multiple cell lines and contexts, introducing Mixscale to improve analysis and infer pathway activation changes.
Nature Cell Biology · Feb 26, 2025Peer-reviewedGenomics