Isabella Mascio

VIPerturb-seq enables genome-wide CRISPR perturbation screens using probe-based detection, improving throughput and efficiency for single-cell analysis.

This paper introduces RNA fingerprinting, a method to map transcriptional responses to perturbations using reference dictionaries, showing accurate single-cell assignments and broad biological applications.

The paper uses Perturb-seq to systematically identify signaling regulator targets across multiple cell lines and contexts, introducing Mixscale to improve analysis and infer pathway activation changes.

The paper introduces CPA-Perturb-seq, a multiplexed perturbation screen dataset, and uses it to identify polyA site regulators and their functional properties.