Gila Lithwick‐Yanai

The paper uses Perturb-seq to systematically identify signaling regulator targets across multiple cell lines and contexts, introducing Mixscale to improve analysis and infer pathway activation changes.

The paper benchmarks PIPseq, a single-cell RNA sequencing method, across different scales and models, showing its ability to detect cell types and differentially expressed genes.

The paper performs genome-scale Perturb-seq to map genotype-phenotype relationships, uncovering new gene functions and stress-specific mitochondrial regulation.

Open flow cell on a circular wafer with mostly natural nucleotides and no reversible terminators: about 300 bp reads at $1/Gb, 99.6% SNP accuracy on reference human genomes.

The paper benchmarks a new low-cost sequencing by synthesis method for scRNA-seq, showing comparable results to existing technologies and enabling cost-effective large-scale applications.