Quantifying HLA transcripts by genotype in chimeric mixtures at single-cell resolution
Sami B. Kanaan, Jason G. Underwood, Rula Green Gladden, Everett Fan, Shruti S. Bhise, Monica S. Thakar, Carla A. Jaeger-Ruckstuhl, Jeffrey Stevens, Ashley Gray, Stanley R. Riddell, Marie Bleakley, Soheil Meshinchi, Scott N. Furlan
This paper introduces scrHLA-typing, a method to quantify HLA transcripts in single cells using long-read sequencing, showing its ability to detect allele-specific expression in post-transplant relapse samples.
bioRxiv · Sep 12, 2025 · PreprintMolecular Biology