Andrew J. Pask

Rapid generation of marsupial induced pluripotent stem cells suitable for use in next-generation conservation

This study generates transgene-free iPSCs from the fat-tailed dunnart, demonstrating their pluripotency and potential for use in conservation biotechnology.

A Cell Viability and Utility Index (CVUI) for wildlife fibroblast biobanking: framework development and preliminary empirical validation

The paper introduces the Cell Viability and Utility Index (CVUI) to assess cell culture quality in wildlife biobanking, validated across 154 culture rounds from 46 species.

Genomes of two arid-zone marsupials uncover contrasting responses to climatic change

The paper generates genome assemblies for two arid-zone marsupials and finds contrasting demographic responses to historical climate changes.

Gene regulatory dynamics during craniofacial development in a carnivorous marsupial

The paper identifies dunnart-specific enhancers and promoters in craniofacial development, showing divergence in regulatory landscapes between marsupials and placental mammals.

Loss-of-function mutations in ASIP and MC1R are associated with coat colour variation in marsupials.

The paper identifies loss-of-function mutations in ASIP and MC1R genes associated with coat color variation in marsupials.

Getting a head start: Craniofacial heterochrony in marsupials involves dynamic changes to molecular and cellular mechanisms underlying neural crest development

This study investigates the cellular and molecular mechanisms of craniofacial heterochrony in marsupials, revealing unique neural crest behaviors that enable accelerated development.

High-Yield Induced Ovulation in Adult Fat-Tailed Dunnarts by PMSG Treatment Combined with Estrus Synchronization

This study develops a protocol to induce high-yield ovulation in adult fat-tailed dunnarts using estrus synchronization and PMSG-hCG treatment, resulting in a high number of mature MII oocytes.

Dissecting functional regulatory convergence over 160 million years of therian evolution

The paper tests regulatory potential of conserved enhancers in thylacine and wolf to assess functional convergence over 160 million years of evolution, finding limited evidence of convergent divergence.

Generation and Assessment of High-Quality Fat-Tailed Dunnart Oocytes Following Superovulation in Prepubertal Animals

This study develops a hormonal regimen to induce ovulation and collect mature oocytes from prepubertal fat-tailed dunnarts, achieving high success rates in oocyte generation and embryo development.

Enrichment of spermatogonial stem cells and staging of the testis cycle in a dasyurid marsupial, the fat-tailed dunnart

This study enriches spermatogonial stem cells in fat-tailed dunnarts using fluorescent dye technology and defines the testis cycle in dasyurids for marsupial conservation research.

Marsupial limb patterning redefines the necessity of lateral plate mesoderm subdivision for limb formation

This study shows that marsupial limb development bypasses lateral plate mesoderm subdivision, challenging previous models of tetrapod limb formation.

Genetically engineered resistance to bufotoxin in marsupial ATP1A1

This study edits the ATP1A1 gene in marsupial cells to confer bufotoxin resistance, showing a >45-fold increase in resistance to bufalin.

De novo transcriptome assembly and genome annotation of the fat-tailed dunnart (Sminthopsis crassicaudata)

The paper presents a de novo transcriptome and improved genome assembly for the fat-tailed dunnart, providing valuable genomic resources for marsupial research.

Genome of the endangered eastern quoll (Dasyurus viverrinus) reveals signatures of historical decline and pelage color evolution.

The paper generates a chromosome-scale reference genome for the eastern quoll and identifies genomic signatures of historical decline and pelage color evolution.

A chromosome-scale hybrid genome assembly of the extinct Tasmanian tiger (Thylacinus cynocephalus)

This paper presents a chromosome-scale hybrid genome assembly for the extinct Tasmanian tiger, providing new genomic resources for marsupial research.

Best Practice Data Life Cycle Approaches for the Life Sciences

The paper provides best practice data life cycle approaches for life sciences researchers focusing on omics datasets and computational data analysis.