Natalie E. Calatayud

The paper introduces the Cell Viability and Utility Index (CVUI) to assess cell culture quality in wildlife biobanking, validated across 154 culture rounds from 46 species.

This study shows that chytridiomycosis infection and heat treatment affect sperm quality in frogs, with severe infection and thermal stress causing lasting reproductive impairments.

The paper introduces the Wildlife Sperm Index (WSI), a standardized scoring system that integrates sperm quality metrics to improve gamete assessment and support assisted reproduction and biobanking in wildlife conservation.

This study develops a non-lethal sperm collection protocol for Rana muscosa, finding that 3 µg/g GnRHa yields the highest sperm quality and Wildlife Sperm Index score.