Robert F. Tesoriero

A Computational Framework for Domain Insertion into Type IV Pili for Bacterial Display and Living Material Assembly

The paper presents a computational framework to predict insertion sites in bacterial Type IV Pili for display, validated with experimental results showing improved display efficiency and surface patterning.

Genetically modifying the protein matrix of macroscopic living materials to control their structure and rheological properties

This study investigates how altering the length of elastin-like polypeptide (ELP) in engineered living materials affects their microstructure and rheological properties, revealing complex sequence-structure-property relationships.

A de novo matrix for macroscopic living materials from bacteria

This paper reports the creation of macroscopic engineered living materials (ELMs) from Caulobacter crescentus cells using a de novo self-interacting protein matrix that can be genetically tuned for mechanical and functional properties.

Engineering high-yield biopolymer secretion creates an extracellular protein matrix for living materials

This paper engineers a two-strain bacterial system to autonomously secrete and assemble a synthetic extracellular protein matrix, demonstrating programmable and high-yield biopolymer secretion.