Charles B. Bott

The paper analyzes metagenomes and metagenome-assembled genomes from microbial communities in a wastewater treatment plant under varying dissolved oxygen conditions to understand microbial adaptation to low-DO environments.

This paper introduces a field-deployable workflow for quantitative metagenomics using Nanopore sequencing and barcoded spike-in calibration, achieving species-level identification and absolute quantitation comparable to digital PCR.

This study demonstrates the feasibility of integrating biological phosphorus removal with anammox-based nitrogen removal in a wastewater treatment process with upstream carbon capture.