Visualizing extracellular vesicle-mediated RNA transfer using a novel metabolic labeling approach
Willemijn S. de Voogt, Tanja V. Edelbacher, Jerney J. Gitz-Francois, Simone M. Smits, Annet C. W. van Wesel, Richard Wubbolts, Pieter Vader
This study introduces a metabolic labeling method using 5-EU and UCK2 overexpression to track RNA in donor cells, EVs, and recipient cells, enabling visualization and quantification of EV-RNA transfer.
bioRxiv · Aug 28, 2025 · PreprintCell Biology