Pieter Vader

Degenerated intervertebral disc environment impairs notochordal cell-derived extracellular vesicles release and their matrix anabolic effect

This study shows that degenerative disc conditions reduce notochordal cell-derived extracellular vesicles release and their ability to promote matrix production in nucleus pulposus cells.

NEO-AAV: an engineered extracellular vesicle-enveloped AAV platform for activation-coupled T cell transduction and CAR-T cell generation

This paper introduces NEO-AAV, an engineered AAV platform that enhances T cell transduction and CAR-T cell generation by enveloping AAV in extracellular vesicles, improving immune resistance and transduction efficiency.

An extracellular vesicle biogenesis-inspired engineering platform for efficient protein delivery and therapeutic base editing

This paper introduces a platform for efficient protein delivery using extracellular vesicles engineered with biogenesis-inspired features, achieving functional gene editing in vitro and in vivo.

A modular strategy for extracellular vesicle-mediated CRISPR-Cas9 delivery through aptamer-based loading and UV-activated cargo release

This paper develops a modular strategy for delivering CRISPR-Cas9 via extracellular vesicles using aptamer-based loading and UV-activated cargo release, demonstrating efficient delivery and versatility in Cas9 variants.

Visualizing extracellular vesicle-mediated RNA transfer using a novel metabolic labeling approach

This study introduces a metabolic labeling method using 5-EU and UCK2 overexpression to track RNA in donor cells, EVs, and recipient cells, enabling visualization and quantification of EV-RNA transfer.

Tissue-specific modulation of CRISPR activity by miRNA-sensing guide RNAs

This paper introduces CRISPR MiRAGE, a miRNA-sensing guide RNA system that enables tissue-specific gene editing by modulating CRISPR activity based on miRNA signatures.

Hypoxia and TNF-alpha modulate extracellular vesicle release from human induced pluripotent stem cell-derived cardiomyocytes

This study investigates how hypoxia and TNF-alpha affect extracellular vesicle release from hiPSC-CMs under stress conditions, identifying nSmase2 and mTOR signaling pathways involved.

Profiling of extracellular small RNAs highlights a strong bias towards non-vesicular secretion

The paper shows that most extracellular miRNAs are secreted non-vesicularly, with only <2% associated with EVs, even though EVs can stabilize miRNAs in serum.