gEL DNA, a cloning- and PCR-free method for CRISPR-based multiplexed genome editing
Paola Randazzo, Nicole Xanthe Bennis, Jean-Marc G Daran, Pascale A. S. Daran-Lapujade
This paper introduces a cloning- and PCR-free method for CRISPR-based multiplexed genome editing in yeast using linear DNA fragments and T7 RNA polymerase.
The CRISPR Journal · Apr 26, 2021Peer-reviewedSynthetic Biology