Pascale A. S. Daran-Lapujade

A protocol for lab-scale production of 13C yeast extract as internal standard for metabolomics and quantification of intracellular metabolites

The paper describes a protocol for producing 13C-labelled yeast extract as an internal standard for metabolomics and quantifying intracellular metabolites.

Assembly and cell-free expression of a partial genome for the synthetic cell

The paper designs and builds a partial synthetic genome that can be expressed in a cell-free system and replicated, demonstrating a proof-of-concept for a minimal synthetic cell.

Exploration of mRNA-sized RNA import into Saccharomyces cerevisiae mitochondria by a combined synthetic biology and adaptive laboratory evolution approach

This study investigates RNA import into yeast mitochondria using synthetic biology and adaptive laboratory evolution, finding that mRNA import remains undemonstrated despite existing short RNA import mechanisms.

Proteome dynamics during transition from exponential to stationary phase under aerobic and anaerobic conditions in yeast

This study quantifies proteome dynamics in yeast during the transition from exponential to stationary phase under aerobic and anaerobic conditions, revealing significant differences in proteome changes between the two environments.

Top-down, knowledge-based genetic reduction of yeast central carbon metabolism

The paper reduces genetic complexity in yeast central carbon metabolism by deleting 35 genes, resulting in minimal phenotypic changes under various conditions.

A systematic evaluation of yeast sample preparation protocols for spectral identifications, proteome coverage and post-isolation modifications

This study evaluates yeast sample preparation protocols for proteomics, finding that urea with filter-aided or in-solution digestion provides best coverage, while solvents like acetone introduce artefacts.

Whole-cell modeling in yeast predicts compartment-specific proteome constraints that drive metabolic strategies

The paper develops a whole-cell metabolic model for yeast to predict compartment-specific proteome constraints that explain metabolic strategies under different growth conditions.

A yeast with muscle does not run faster: full humanization of the glycolytic pathway in Saccharomyces cerevisiae

This study successfully humanizes the entire glycolytic pathway in yeast, revealing conserved functions and context-dependent responses.

gEL DNA, a cloning- and PCR-free method for CRISPR-based multiplexed genome editing

This paper introduces a cloning- and PCR-free method for CRISPR-based multiplexed genome editing in yeast using linear DNA fragments and T7 RNA polymerase.

A supernumerary designer chromosome for modular in vivo pathway assembly in Saccharomyces cerevisiae

This paper introduces synthetic chromosomes in yeast for modular pathway engineering, demonstrating efficient assembly and stable replication of non-coding DNA.