Xinyi Guo

The paper develops an automated pipeline to design CRISPR prime editors for human pathogenic variants, finding that only a small fraction can be targeted but this number increases to over 50,000 with alternative Cas9 and templates.

The paper develops a computational model to optimize Cas13 guide RNA designs for efficient RNA knock-down, validated across endogenous targets and providing a genome-wide resource for CRISPR screens.