Mateusz Legut

A universal platform for simultaneous TCRα/β removal enables safer and more potent TCR therapies and autoimmune modeling

This paper presents a CRISPR-based platform that removes endogenous TCRα/β chains to enhance transgenic TCR function and safety in T-cell therapies and autoimmune modeling.

Paired CRISPR screens to map gene regulation in cis and trans

The paper uses paired CRISPR screens to identify cis- and trans-regulatory elements controlling PD-L1 expression in PDAC cells and links them to clinical outcomes.

Expanding the scope of bacterial CRISPR activation with PAM-flexible dCas9 variants

This study evaluates PAM-flexible dCas9 variants for bacterial CRISPR activation and repression, finding trade-offs between PAM flexibility and repression efficiency.

Principles for rational Cas13d guide design

The paper develops a computational model to optimize Cas13 guide RNA designs for efficient RNA knock-down, validated across endogenous targets and providing a genome-wide resource for CRISPR screens.

Expanding the CITE-seq tool-kit: Detection of proteins, transcriptomes, clonotypes and CRISPR perturbations with multiplexing, in a single assay

The paper introduces ECCITE-seq, a method that simultaneously captures transcriptomes, immune clonotypes, surface markers, sample identity, and CRISPR perturbations from single cells.