Alejandro Méndez‐Mancilla

Low copy CRISPR-Cas13d mitigates collateral RNA cleavage

The paper evaluates collateral RNA cleavage in CRISPR-Cas13d systems and finds that low-copy expression reduces off-target effects while maintaining on-target knockdown.

Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq

The paper introduces CaRPool-seq, a method using Cas13 for combinatorial RNA perturbations in single cells, and applies it to study myeloid differentiation regulators in AML.

Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens

The paper predicts Cas13 guide RNA efficacy for transcripts in model organisms and viral RNA pathogens and provides a web tool for gRNA design.

Scalable pooled CRISPR screens with single-cell chromatin accessibility profiling

This paper introduces CRISPR-sciATAC, a method linking genetic perturbations to chromatin accessibility in single cells, showing its effectiveness in human and mouse experiments.

Principles for rational Cas13d guide design

The paper develops a computational model to optimize Cas13 guide RNA designs for efficient RNA knock-down, validated across endogenous targets and providing a genome-wide resource for CRISPR screens.