Removing auto-activators from yeast-two-hybrid assays by conditional negative selection
Devendra Shivhare, Magdalena Musialak‐Lange, Irene Julca, Pawel Gluza, Marek Mutwil
The paper develops a negative selection method to remove auto-activators from yeast-two-hybrid assays using pGAL2-URA3 and 5-FOA treatment, reducing false-positive hits.
Scientific Reports · Mar 9, 2021Peer-reviewedMolecular Biology