Donald Hilvert

This paper designs de novo ligases that achieve site-specific ubiquitylation of a target protein with high selectivity and efficiency.

This study designs de novo amide ligases with a cysteine catalytic triad to selectively form amide bonds across diverse substrates, demonstrating high selectivity and applicability in synthesizing bioactive molecules.

This study designs de novo cysteine proteases with high activity and structural novelty, achieving 19% success rate and up to 3 x 10^7 rate enhancement over background reactions.

This paper designs de novo metalloproteases using RoseTTAFold Diffusion 2, achieving high activity and specificity for cleaving various substrates.

The paper demonstrates splitting a 240-subunit nucleocapsid into two fragments to create a 480-subunit two-component capsid with new surface sites for functionalization.

This paper designs highly active metallohydrolases using a new generative AI method, achieving kcat/KM values up to 53,000 M-1 s-1 without experimental optimization.