Kejia Wu

MYC targeted de novo binders impair tumor growth

Designed protein binders to conserved MYC regions reach low-nanomolar affinity, reduce MYC-MAX proximity in cells, and are depleted from MYC-dependent cancer cells in culture and mouse pancreatic tumors.

Design of readers for pathological PTM proteoforms in Alzheimer's disease

The paper designs and validates protein readers that specifically detect disease-related modifications in Tau and Abeta in Alzheimer's disease models and human samples.

Design of synthetic activators for programmable gene expression

This study designs synthetic activators for programmable gene expression by engineering coactivator-binding proteins and demonstrates their enhanced transcriptional activation capabilities.

Computational design of metalloproteases

This paper designs de novo metalloproteases using RoseTTAFold Diffusion 2, achieving high activity and specificity for cleaving various substrates.

De novo design of Ras isoform selective binders

This study designs Ras isoform-specific binders using deep learning to target the C-termini of Ras proteins, enabling specific inhibition and study of their roles in biology and disease.

De novo design of phosphotyrosine peptide binders

This paper introduces RFD2-MI, a deep learning framework for designing phosphotyrosine peptide binders, showing high specificity and affinity for phosphorylated targets.

Diffusing protein binders to intrinsically disordered proteins

The paper develops RFdiffusion to generate protein binders for IDPs/IDRs, achieving nanomolar affinity and functional applications in amylin and IL2RG.

Design of intrinsically disordered region binding proteins

The paper designs proteins that bind intrinsically disordered regions with high affinity and specificity across diverse targets.

Multivalent designed proteins protect against SARS-CoV-2 variants of concern

The paper designs and characterizes trivalent miniprotein binders that neutralize SARS-CoV-2 variants with low pM IC50 values and provide prophylactic and therapeutic protection in mice.