Deepika Gaur

ALS mutations disrupt self-association between the Ubiquilin Sti1 hydrophobic groove and internal placeholder sequences

This study presents the first crystal structure of the Ubiquilin Sti1 domain bound to a transmembrane domain, showing that ALS mutations disrupt their interaction and reveal internal placeholder sequences that regulate phase separation.

An Msp1-Protease Chimera Captures Transient AAA+ Interactions and Unveils Ost4 Mislocalization Errors

This study creates an Msp1-protease chimera to capture transient AAA+ interactions, identifies Ost4 as a novel Msp1 substrate, and reveals mislocalization errors in Ost4.

A suite of pre-assembled, pET28b-based Golden Gate vectors for efficient protein engineering and expression

The paper develops a suite of pre-assembled Golden Gate vectors for efficient protein expression and purification in E. coli, enabling customizable and high-throughput protein engineering.

Energetic requirements and mechanistic plasticity in Msp1-mediated substrate extraction from lipid bilayers

This study investigates how Msp1 uses ATP hydrolysis to extract transmembrane helices from lipid bilayers, finding a non-linear relationship between ATP hydrolysis and extraction efficiency.