Shilpi Agrawal

Beyond the Cut: Long-read sequencing reveals complex genomic and transcriptomic changes in AAV-CRISPR therapy for Duchenne Muscular Dystrophy

This study uses long-read sequencing to analyze genomic and transcriptomic changes in AAV-CRISPR therapy for DMD, revealing complex editing outcomes and developing new methods for detection.

Standardizing a Protocol for Streamlined Synthesis and Characterization of Lipid Nanoparticles to Enable Preclinical Research and Education

This paper develops a standardized, cost-effective microfluidic LNP synthesis protocol with reproducible results across user groups and lipid types.

A Dual-Fluorescence Assay for Gene Delivery Vehicle Screening in Macrophages with an Inflammation-Inducible Reporter Construct

The paper develops a dual-fluorescence assay to screen gene delivery vehicles in macrophages using an inflammation-inducible reporter, showing divergent effects of different delivery methods.

Precision and efficacy of RNA-guided DNA integration in high-expressing muscle loci

This study evaluates CRISPR-based gene integration in skeletal muscle using two high-expressing loci, demonstrating high-level therapeutic gene expression in mouse myoblasts.