CRISPR-Cas9 trans-cleavage is hindered by a closed R-loop, an elongated spacer, and an inactive HNH domain
Roser Montagud‐Martínez, Raúl Ruiz, Sara Baldanta, Rubén Delicado-Mateo, Guillermo Rodrigo
The paper identifies structural factors affecting CRISPR-Cas9 trans-cleavage activity, including R-loop conformation, spacer length, and HNH domain activity.
bioRxiv · Apr 17, 2025 · PreprintBiochemistry