Author

Raúl Ruiz

Universitat de ValènciaORCID 0000-0002-5300-2240Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

Click to filter author papers

Biochemistry· 1 Microbiology· 1 Molecular Biology· 1 Synthetic Biology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Research Focus:database / resource· 2 Approach:experimental assay· 2 Approach:statistical model· 2 Assay:DNA methylation· 1 Organism:E. coli· 1 Research Focus:gene regulatory network inference· 1 Research Focus:genome assembly· 1 Assay:metagenomics· 1

Frequent Co-Authors

Belén Esquerra-RuviraiD· 2 shared Francisco J. M. MojicaiD· 2 shared Guillermo RodrigoiD· 2 shared Roser Montagud‐MartíneziD· 2 shared Alejandro Requena-MenéndeziD· 1 shared Almudena FernándeziD· 1 shared Anna Pérez‐RàfolsiD· 1 shared Carlos Manuel DuarteiD· 1 shared

Activity by Year

2023-2025 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 18 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

2 shared papersMicrobiology

Belén Esquerra-RuviraORCID

Mutual co-authors in cluster

Author profile →

4 papers

Recent·Most cited

Widespread and Abundant CRISPR-Cas Systems in the Deep Ocean

Pablo Sánchez, Marta Ferri-Peradalta, Ester María López-García, Raúl Ruiz, Belén Esquerra-Ruvira, Josep M. Gasol, Dolors Vaqué, Takashi Gojobori, Susana Agustı́, Carlos Manuel Duarte, Francisco J. M. Mojica, Julián Cerón, Felipe H. Coutinho, Silvia G. Acinas

The paper identifies a high abundance of CRISPR-Cas systems in deep ocean microbiomes, highlighting their diversity and potential for bioprospecting.

bioRxiv · May 26, 2025 · PreprintMicrobiology

CRISPR-Cas9 trans-cleavage is hindered by a closed R-loop, an elongated spacer, and an inactive HNH domain

Roser Montagud‐Martínez, Raúl Ruiz, Sara Baldanta, Rubén Delicado-Mateo, Guillermo Rodrigo

The paper identifies structural factors affecting CRISPR-Cas9 trans-cleavage activity, including R-loop conformation, spacer length, and HNH domain activity.

bioRxiv · Apr 17, 2025 · PreprintBiochemistry

Repurposing the mammalian RNA-binding protein Musashi-1 as an allosteric translation repressor in bacteria

Roswitha Dolcemascolo, María Heras‐Hernández, Lucas Goiriz, Roser Montagud‐Martínez, Alejandro Requena-Menéndez, Raúl Ruiz, Anna Pérez‐Ràfols, R. Anahí Higuera-Rodríguez, Guillermo Pérez-Ropero, Wim Vranken, Tommaso Martelli, W. Kaiser, Jos Buijs, Guillermo Rodrigo

This paper engineers a bacterial gene regulation system using the mammalian RNA-binding protein Musashi-1 to repress translation through an allosteric mechanism.

eLife · Oct 12, 2023Peer-reviewedSynthetic Biology

Identification of the EH CRISPR-Cas9 system on a metagenome and its application to genome engineering

Belén Esquerra-Ruvira, Ignacio Baquedano, Raúl Ruiz, Almudena Fernández, Lluı́s Montoliu, Francisco J. M. Mojica

The paper identifies a new CRISPR-Cas9 system from a metagenome, characterized by a smaller Cas9 protein (EHCas9) with DNA targeting capabilities, and develops a simplified tool for genome editing in prokaryotes and eukaryotic cells.

Microbial Biotechnology · Apr 25, 2023Peer-reviewedMolecular Biology