Guillermo Rodrigo

This paper develops an amplification-free CRISPR-Cas13a platform for direct viroid detection in plant RNA extracts, demonstrating its effectiveness for multiple viroid families.

The paper identifies FeSOD1 as a Cu-deficiency-induced interactor of AGO1 that protects it from oxidative stress, preserving miRNA-mediated gene silencing.

The paper identifies structural factors affecting CRISPR-Cas9 trans-cleavage activity, including R-loop conformation, spacer length, and HNH domain activity.

This paper engineers a bacterial gene regulation system using the mammalian RNA-binding protein Musashi-1 to repress translation through an allosteric mechanism.

This paper designs and characterizes RNA circuits that dynamically process signals to control gene expression in live cells.

The paper designs cooperative riboregulation in E. coli using a hierarchical toehold activation cascade and a de novo sequence design algorithm.

This study engineers a circular riboregulator in E. coli using group I PIE sequences, showing increased gene expression activation compared to linear forms.

This paper presents an evolutionary computation method combined with strand displacement techniques to systematically design riboregulators for genetic control systems in bacteria.