Atsushi Shibai

Closed-loop steering locates an evolutionary barrier to multidrug resistance in Escherichia coli

The paper uses a closed-loop system to steer Escherichia coli toward multidrug resistance and identifies an evolutionary barrier linked to mutations in the efflux pump gene.

Laboratory evolution of the bacterial genome structure through insertion sequence activation

This study accelerates bacterial genome evolution by introducing multiple insertion sequences into E. coli, resulting in significant genome rearrangements and size changes.