The long and the short of it: Nanopore based eDNA metabarcoding of marine vertebrates works; sensitivity and specificity depend on amplicon lengths
Karlijn Doorenspleet, Max Janse, Saskia Oosterbroek, Pauline Kamermans, O.G. Bos, Erik Wurz, Albertinka Jacoba Murk, Reindert Nijland
This paper compares nanopore-based eDNA metabarcoding using 2kb and 170bp amplicons for marine fish species identification, finding that longer amplicons improve taxonomic resolution in controlled settings but yield fewer species in field samples.
Molecular Ecology Resources · Feb 10, 2025Peer-reviewedMolecular Biology